US 12264358

Summary of U.S. Patent 12,264,358

A search of the United States Patent and Trademark Office (USPTO) database and the Court of Appeals for the Federal Circuit (CAFC) 2026 dockets for patent number 12264358 did not yield any direct results. The following summary is based on the authoritative text provided.

Title: Method of selectively sequencing amplicons in a biological sample

Assignee: Harvard University (originally assigned to President and Fellows of Harvard College)

Inventors:

• George M. Church
• Jehyuk Lee
• Richard C. Terry
• Evan R. Daugharthy

Filing Date: May 2, 2024

Issue Date: April 1, 2025

Abstract:
Methods of selectively sequencing amplicons in a biological sample are provided.

Plain-Language Overview of Independent Claims:
The provided text for US Patent 12,264,358 does not include the specific claims of the patent. Therefore, a plain-language overview of the independent claims cannot be provided.

The patent generally describes methods for creating a three-dimensional matrix of nucleic acids (DNA or RNA) and then amplifying, detecting, and sequencing these nucleic acids directly within that matrix. A key aspect is the ability to perform these steps in situ, meaning within their original context, such as inside a cell or tissue sample. This preserves the spatial orientation of the nucleic acids, providing information about their location. The invention allows for the nucleic acids to be fixed in place by being covalently bound to a porous, optically transparent matrix material, such as a polyacrylamide gel. This stable structure allows for repeated cycles of sequencing and imaging, effectively turning the biological sample into a stable information storage medium that can be read multiple times.