US 7622281

Patent Overview — US7622281B2

Title: Methods and compositions for clonal amplification of nucleic acid

Patent number / application: US7622281B2; application US11/134,683

Assignee: Leland Stanford Junior University (original assignee; an assignment to "Board of Trustees of the Leland Stanford Junior University, The" is recorded 2005-10-14)

Key dates (as listed): Priority date 2004-05-20; filing date 2005-05-19; publication of US20060008824A1 2006-01-12; granted 2009-11-24. Legal status shown as "Expired – Fee Related," adjusted expiration 2026-01-20.

Inventors: Mostafa Ronaghi and Foad Mashayekhi.

Purpose: The text frames the invention against the Human Genome Project's cost and throughput limits, noting that further cost reductions of two or three orders of magnitude would require a highly integrated platform. The invention "relates to labelling nucleic acid molecules with a nucleic acid barcode, which enables easy identification of the nucleic acid after amplification and sequencing of the barcoded nucleic acid molecule."

Plain-language scope (per the excerpt): A unique primer—an adapter containing a unique barcode, preferably a five-nucleotide sequence—is attached (e.g., ligated) to a nucleic acid molecule to identify its source (such as a BAC clone, bacterium, or tissue sample). The method amplifies the labeled molecule, including by sequencing-by-synthesis, pyrosequencing, massively parallel signature sequencing, BEAM, or polony technology. One recited method involves hybridizing the labeled molecule to immobilized anti-primers on a solid surface (e.g., a magnetic or Sepharose bead), extending with polymerase and nucleotides, denaturing, re-extending with labeled nucleotides and hybridizing oligonucleotides, optionally immobilizing additional anti-primers, and sequencing the immobilized amplified molecules. The excerpt also recites claims to nucleic acid molecules attached to solid surfaces, solid surfaces bearing such molecules, and kits containing a unique primer, anti-primer-coated surface, oligonucleotides, and instructions. Figures 1–2 schematically depict barcoded clonal amplification and clonal amplification.

Source: https://patents.google.com/patent/[US7622281](/patent/US7622281)/en

What the excerpt does not establish: It does not provide the full claim set, the complete written description, or the exact wording of granted claims. It does not establish scope of claim coverage, patentability, validity, enforceability, or prior art. It does not establish current ownership, licensing, or litigation status.